Mirus Evo is a patented, precision, microfluidic, 8-channel syringe pump for cell analysis under shear flow mimicking physiological flow in the human vasculature.
Includes MultiFlow8, a manifold that enables the flow from the Mirus Evo to be split equally into 8 separate channels to conduct 8 assays simultaneously in the Vena8 range of biochips (or your custom biochips), resulting in higher throughput with this 8-channel syringe pump. Mirus Evo is PC- controlled by VenaFluxAssay software.
Mirus Evo Pump Quick-Start Guide
In this blog we will explain how to get your Mirus Evo pump up and running by simply following the steps below :
First we will look at connecting the syringe to the Mirus Evo Pump
Then we will look at tubing for the pump
After that we will look at setting up the MultiFlow8
Then connecting the MultiFlow8 to the Mirus Evo Pump
We will look at connecting a reservoir to the pump
Before finally discussing power supply and communications
1. Connecting the Syringe to the Mirus Evo pump
First, make sure the pump is turned off
Next, Connect your syringe to the drive valve, and screw in the syringe
Bring the plunger down into the plunger lock screw, and
Screw in the plunger lock screw
Check to make sure that everything’s tightened and fixed
2. Setting up the Tubing
There are three cables : Orange, blue and green
The orange cable is the input cable, it connects reservoir to Mirus Evo pump
The blue cable connects the Mirus Evo pump to the pump manifold
And the green cable is the output of the manifold, and connects to the MultiFlow8, which is explained further in the next steps
The media flows from the reservoir through the orange cable to the Mirus Evo pump, then flows through the blue cable to the pump manifold, before finally flowing through the green cable out to the MultiFlow8
3. Setting up the MultiFlow8
First, we want to set up the 8-way cable which allows us to connect the MultiFlow8 to a microfluidic chip
To do this, first screw the 8 nozzles into the MultiFlow8 by hand,
Then, using an appropriate tool, secure the 8 nozzles by screwing them each in by a quarter turn
Then, with the 8-way tubing, plug in each tube to the corresponding number on the MultiFlow8
This means you take the end tube of the 8-way cable, and plug it into the nozzle labelled 1, then you take the next tube along, and plug it into the nozzle labelled 2 and so on until all tubes are connected
4. Connecting the MultiFlow8 to the Mirus Evo pump
Take the stopcock T junction, and screw it into the MultiFlow8
This allows us to connect the output tube of the Mirus Evo to the MultiFlow8, and also later allows us to add a syringe to the top junction for a quicker washout later
To connect the Mirus Evo to the MultiFlow8, plug the green cable into the side junction, and screw it in
5. Connecting the reservoir
Now we can connect a reservoir to the Mirus Evo pump. To do this, simply insert the orange cable into the reservoir.
6. To finish the setup, let us take a look at Power supply and Communications
There are 3 connections on the Mirus Evo to think about: the power connection, the usb connection, and the connection to the MultiFlow8
First, plug in the power cable, ensuring that the pins on the connectors line up
Next plug in the USB connection. This will connect to your PC
Finally, to connect the MultiFlow8, take the black tipped end of the orange cable and slide it back and hold it back before connecting it to the Mirus Evo
The other right angle end of the cable connects to the bottom of the MultiFlow8. Making sure the pins line up, connect the cable and screw it in.
So now you have set up your Mirus Evo pump and the MultiFlow8, and you know how each piece works.
But, if you still have questions or want to learn more, check out our website, at wearecellix.com, or contact us @info@wearecellix.com