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How to get started with your Mirus Evo 8-channel Microfluidic Syringe Pump

Mirus Evo is a patented, precision, microfluidic, 8-channel syringe pump for cell analysis under shear flow mimicking physiological flow in the human vasculature.

Includes MultiFlow8, a manifold that enables the flow from the Mirus Evo to be split equally into 8 separate channels to conduct 8 assays simultaneously in the Vena8 range of biochips (or your custom biochips), resulting in higher throughput with this 8-channel syringe pump. Mirus Evo is PC- controlled by VenaFluxAssay software.

Mirus Evo Pump Quick-Start Guide

In this blog we will explain how to get your Mirus Evo pump up and running by simply following the steps below :

  • First we will look at connecting the syringe to the Mirus Evo Pump

  • Then we will look at tubing for the pump

  • After that we will look at setting up the MultiFlow8

  • Then connecting the MultiFlow8 to the Mirus Evo Pump

  • We will look at connecting a reservoir to the pump

  • Before finally discussing power supply and communications

1. Connecting the Syringe to the Mirus Evo pump

  • First, make sure the pump is turned off

  • Next, Connect your syringe to the drive valve, and screw in the syringe

  • Bring the plunger down into the plunger lock screw, and

  • Screw in the plunger lock screw

  • Check to make sure that everything’s tightened and fixed

2. Setting up the Tubing

  • There are three cables : Orange, blue and green

  • The orange cable is the input cable, it connects reservoir to Mirus Evo pump

  • The blue cable connects the Mirus Evo pump to the pump manifold

  • And the green cable is the output of the manifold, and connects to the MultiFlow8, which is explained further in the next steps

  • The media flows from the reservoir through the orange cable to the Mirus Evo pump, then flows through the blue cable to the pump manifold, before finally flowing through the green cable out to the MultiFlow8

3. Setting up the MultiFlow8

  • First, we want to set up the 8-way cable which allows us to connect the MultiFlow8 to a microfluidic chip

  • To do this, first screw the 8 nozzles into the MultiFlow8 by hand,

  • Then, using an appropriate tool, secure the 8 nozzles by screwing them each in by a quarter turn

  • Then, with the 8-way tubing, plug in each tube to the corresponding number on the MultiFlow8

  • This means you take the end tube of the 8-way cable, and plug it into the nozzle labelled 1, then you take the next tube along, and plug it into the nozzle labelled 2 and so on until all tubes are connected

4. Connecting the MultiFlow8 to the Mirus Evo pump

  • Take the stopcock T junction, and screw it into the MultiFlow8

  • This allows us to connect the output tube of the Mirus Evo to the MultiFlow8, and also later allows us to add a syringe to the top junction for a quicker washout later

  • To connect the Mirus Evo to the MultiFlow8, plug the green cable into the side junction, and screw it in

5. Connecting the reservoir

Now we can connect a reservoir to the Mirus Evo pump. To do this, simply insert the orange cable into the reservoir.

6. To finish the setup, let us take a look at Power supply and Communications

  • There are 3 connections on the Mirus Evo to think about: the power connection, the usb connection, and the connection to the MultiFlow8

  • First, plug in the power cable, ensuring that the pins on the connectors line up

  • Next plug in the USB connection. This will connect to your PC

  • Finally, to connect the MultiFlow8, take the black tipped end of the orange cable and slide it back and hold it back before connecting it to the Mirus Evo

  • The other right angle end of the cable connects to the bottom of the MultiFlow8. Making sure the pins line up, connect the cable and screw it in.

So now you have set up your Mirus Evo pump and the MultiFlow8, and you know how each piece works.

But, if you still have questions or want to learn more, check out our website, at, or contact us


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