VenaFlux : Accessories required to conduct a thrombosis assay using the VenaFlux setup

Quantify cell adhesion, rolling, migration, cell-cell adhesion, platelet aggregation, thrombi formation - all under shear flow.

Introduction

The circulatory system contains many different types of blood vessels which delivers blood cells, nutrients, oxygen, carbon dioxide and hormones to all parts of the body. Blood vessels play a huge role in many medical conditions such as:

Thrombosis
Inflammation
Atherosclerosis

VenaFlux Solutions comprise packages to suit all budgets, with our Starter kit including a precision microfluidic pump, a microenvironmental chamber to hold the biochips which contain engineered microcapillaries and cell analysis software; to Pro and Elite versions including high-end fluorescent microscopes with a digital camera. The VenaFluxAssay software integrates the microfluidic pump, microscope and camera resulting in a complete automated high-throughput flow assay system for shear stress experiments.

Features and Benefits

COST EFFECTIVE SOLUTION - integrate with your own inverted microscope.
THROMBOSIS, PLATELET ADHESION & AGGREGATION ASSAYS: 2.25 - 450 dyne/cm with Vena8 Fluoro+ Biochips.
CELL-LIGAND & CELL-CELL ROLLING, ADHESION & MIGRATION SHEAR FLOW ASSAYS: 0.05 - 10 dyne/cm with Vena8 Fluoro+ and Vena8 Endothelial+ Biochips.
MULTIPLEXED ASSAYS with Mirus Evo Pump and MultiFlow8 providing equal flow rates in 8 channels of Cellix's biochips. This enables multiple cell types or adhesion molecules to be tested simultaneously comparing different therapeutic treatments.
TEMPERATURE CONTROLLED CONDITIONS mimicking physiological conditions with microenvironmental chamber.
CELL ANALYSIS of % thrombus coverage and number of thrombi; number of cells adhered to protein-coated channels and analysis of rolling velocities of cells.
PROGRAMMABLE with PC-controlled VenaFluxAssay software enabling execution of customised protocols.

VenaFlux Solutions

VenaFlux Solutions comprise packages to suit all budgets. The four packages are as follows:

What accessories do I require to conduct a thrombosis assay with my VenaFlux set-up?

Below is a list of additional items required to conduct a thrombosis assay with the VenaFlux:

A. Eppendorf Holder

This sits on the microscope stage to hold the regular Eppendorf tubes.
Fits onto standard frame
Size (L x W x H): 4.7cm x 2.4cm x 3.2c
Net Weight (kg): ~0.03kg per unit
Delivery: 2-4 weeks

B. Humidified Box

This is used to store the biochips during coating with VWF, fibrinogen, collagen etc.
Prevents evaporation of adhesion molecules coated onto biochips
Size (L x W x H): 11cm x 12.5cm 6.9cm
Net Weight (kg): ~0.21kg per unit
Delivery: 2-4 weeks

C. Biochip Single Outlet Tube

These are used to connect from the output of the Vena8 Fluoro+ biochip (via the stainless steel tube) to the Eppendorf which is placed in the Eppendorf Holder.
Size (L x W x H): Pouch size: 9cm x 13cm
Net Weight (kg): ~0.01kg per unit
Delivery: ~2-4 weeks.

D. Reagents

Main reagents used in thrombosis:

Collagen fibrils (type I)
Von Willebrand factor (VWF)
Fibrinogen

This will depend on the customer's experiment. Usually for thrombosis, the customer will pre-coat the biochip with a reagent like VWF or Collagen. We can help with this when we go through the training with them.

The products mentioned above are available on our eShop. If you have any questions just drop us a mail at info@wearecellix.com. All our products are customisable to the needs of our customers. Talk to us about solutions that serve your goals best.