Introduction
Mirus Evo is a patented, precision, microfluidic, 8-channel syringe pump for cell analysis under shear flow mimicking physiological flow in the human vasculature. Includes MultiFlow8, a manifold that enables the flow from the Mirus Evo to be split equally into 8 separate tubes to conduct 8 assays simultaneously in the Vena8 range of biochips, resulting in higher throughput with this 8-channel syringe pump. Mirus Evo is PC- controlled by VenaFlux Assay software. In this article , we will explain how to set up the Mirus Evo Pump, and the Multiflow8.
Step 1
Connecting the syringe to the Mirus Evo Pump
Next, we'll proceed to discuss tubing options for the pump. Following that, we'll delve into setting up the Multiflow8. Finally, we'll connect the Multiflow8 to the Mirus Evo Pump.
We'll look at connecting a reservoir to the pump, before finally discussing power supply and communications. To start, we’ll look at Connecting the Syringe to the Mirus Evo pump.
First, make sure the pump is turned off
Next, Connect your syringe to the drive valve, and screw in the syringe.
Bring the plunger down into the plunger lock screw, and
Screw in the plunger lock screw.
Check to make sure that everything’s tightened and fixed.
Step 2
Setting up the tubing for the pump
There are three cables in our setup. The first is the orange cable, which serves as the input, facilitating the connection between the reservoir and the Mirus Evo pump. Following this, the blue cable links the Mirus Evo pump to the pump manifold. Finally, the green cable functions as the output of the manifold, establishing a connection with the Multiflow8 device, which we'll discuss in more detail shortly.
The media flows from the reservoir through the orange cable to the mirus evo pump, then flows through the blue cable to the pump manifold, before finally flowing through the green cable out to the multiflow8.
Step 3
Connecting the Multiflow8 to Mirus Evo
First, we want to set up the 8-way cable which allows us to connect the multiflow8 to a microfluidics chip.
To do this, first screw the 8 nozzles into the multiflow8 by hand,
Then, using an appropriate tool, secure the 8 nozzles by screwing them each in by a quarter turn
Then, with the 8-way tubing, plug in each tube to the corresponding number on the multiflow8.
This means you take the end tube of the 8-way cable, and plug it into the nozzle labelled 1. Then you take the next tube along, and plug it into the nozzle labelled 2, and so on until all tubes are connected.
Take the stopcock T junction, and screw it into the multiflow8.
This allows us to connect the output tube of the mirus evo to the multiflow8, and also later allows us to add a syringe to the top junction for a quicker washout later.
To connect the Mirus Evo to the multiflow8, plug the green cable into the side junction, and screw it in.
Step 5
Connecting an inlet cable from a reservoir to the Mirus Evo Pump
To do this, simply insert the orange cable into the reservoir.
Step 6
To finish the setup, we’ll look at Power supply and Communications.
There are 3 connections on the mirus evo to think about: the power connection, the usb connection, and the connection to the multiflow8.
First, plug in the power cable, ensuring that the pins on the connectors line up.
Next plug in the USB connection. This will connect to your PC.
Finally, to connect the multiflow8, take the black tipped end of the orange cable and slide it back and hold it back before connecting it to the Mirus Evo.
The other right angle end of the cable connects to the bottom of the Multiflow8. Making sure the pins line up, connect the cable and screw it in.
So now you’ve set up your Mirus Evo pump and the multiflow8, and you know how each piece works.
But, if you still have questions or want to learn more, check out our website, at wearecellix.com, or contact us here.