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All you need to know about your 4U Pump

4U microfluidic pump is a 4-channel precision pressure pump for a variety of microfluidic applications, where accurate and stable flow rate delivery is required. All the features of the pump can be controlled with the SmartFlo software running on your PC.


  • FAST RESPONSE and fast settling time.

  • PULSE-FREE down to µL/min with flow sensor.

  • PROGRAMMABLE flow profiles with any combination of constant, ramp, sine and step.

  • EASY INTEGRATION with your own software - LabVIEW, C++, Python, etc.

  • FLOW RATES: 1µL/min - 1mL/min.

  • INPUT PRESSURE: up to 10 bar or 145 PSI - external compressor or vacuum pump required.

  • CLOSED-LOOP FEEDBACK: Flow sensors provide active feedback and precise flow control.

  • EASY TO USE: simple software control via SmartFlo software.


  • Droplet Generation: high throughput droplet micro-reactor generation.

  • Hydrodynamic flow focusing

  • Organ-on-chip experimental set-ups

  • Volatile Organic Compound (VOC) experimental set-ups​

  • Shear based cell rolling, adhesion and transmigration assays on ligand-coated surface or on endothelial cells.​

  • Precise mixing of soluble compounds in microchannels.

  • Bacterial biofilm formation on solid surfaces


Can the 4U Pump be used for Droplet Generation experiments (single and double encapsulation)?

✅ Yes, one of the applications of our 4U Pumps is Droplet Generation. Below is a video on how to use 4U Pump for Droplet Generation:

Can the 4U Pump be used for long term cell culture and Organ-on-chip experiments?

✅ Yes, one of the applications of our 4U Pumps is Organ-on-chip experiments. Below is a video on how to use 4U Pump for Organ-on-chip studies:

Can two 4U Pumps be used simultaneously while using the SmartFlo software?

✅ Currently, the 4U Pump is optimised to be used individually but we would be happy to customise the pump as per your needs and requirements.

Can you use the 4U Pump with your existing flow chambers and tubing kits?

✅ Yes, 4U Pumps are compatible with different flow chambers and tubing. However, if you require tubing and flow chambers, we would be happy to provide them. Check them out on our eShop.

Can the 4U Pump be used in vacuum mode?

✅ Yes, 4U Pumps can be used in vacuum (or "pull") mode.

Can you have independent flow control in the 4U Pump?

✅ Yes, the flow control in the 4U Pump is completely customisable. Below is a video on how to customise flow control:


How do you pull out the air hose

✅ Please, do not force it. It can damage the pump. You must press on the edge of the fluidic adaptor it is connected to, and then pull it out slowly.

Pressure is building slowly, and flow rate does not reach preset level

✅ Please, check if all the fluidic connections are tightened properly so they are not leaking air. Ensure you also check connections to the sample reservoirs and flow sensors.

Pressure is not building

✅ Make sure that the external air compressor is connected and switched on.

Pump is not powering up

✅ Please, press the power button and hold it for 2-3 seconds until it turns green. If you press the button but do not hold it pressed, the pump will not power up. This is the same for powering down the pump.

❓Pressure line hose does not fit

✅ Make sure that the pressure line hose is 6mm in diameter. If your external pressure compressor has a bigger or smaller pressure line hose, you will need a pneumatic adapter to 6mm. These are widely available.

These are some of the most common queries we have for our 4U Microfluidic Pumps, but if you have any questions just drop us a mail at All our products are customisable to the needs of our customers. Talk to us about solutions that serve your goals best.

You can also check out our eShop.


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