Kima Pump : Recirculating pump for Cell Culture in Flow Chambers


Kima pump is a microfluidic pump designed to aid cell culture (e.g. endothelial cells) under flow in various biochips and flow chambers, including Cellix’s Vena8 Endothelial+ biochips where it is possible to culture 8 cell monolayers simultaneously over 24–48 hours. Cell culture can be continued for up to several weeks if required. The Kima pump is controlled by the iKima Application on the iPod Touch and iPhone. The Kima pump is placed inside a standard incubator while the iPod Touch or iPhone, as it’s controller, sits outside the incubator. The Kima pump connects easily to Cellix’s Vena8 Endothelial+ biochip and other manufacturer’s flow chambers via fully autoclavable tubing and delivers pulses of fresh media to cells seeded inside the microchannels of the biochip. The iKima App is available for download from Apple’s iTunes store.


Features and Benefits :

  • Cell Culture under flow: simple culture of endothelial cell monolayers under flow in flow chambers and 8 cell monolayers in Cellix's Vena8 Endothelial+ biochips within 24-48 hours.

  • Flow Rates: ~250 - 600µL/min. If using Kima pump with Vena8 biochip range, the flow splitter will result in flow rates of ~30 - 75µL/min in each of the 8 channels of the biochip.

  • ​Recirculating Culture Media: saves reagents and no additional fluidic valves required.

  • Incubator Safe: No additional temperature or humidity control required.

  • Pulsatile: apply continuous or periodic pulses of fresh culture media to cell monolayers in culture.

  • Programmable: with iPod Touch.

  • Easy to Use: control up to 4 pumps with the same controller and iPod Touch.

Applications :

  • Cell culture of endothelial cell monolayers for shear flow experiments.​

  • Cell-cell adhesion shear flow assays on endothelial cell monolayers with Cellix's Mirus Evo Nanopump or ExiGo pump for studies such as:

  1. Cardiovascular / Atherosclerosis​: primary monocyte adhesion to coronary artery endothelial cells.

  2. Inflammation​: THP-1 monocyte adhesion to HUVECs under flow.

  3. Asthma / COPD: eosinophil adhesion studies to human lung microvascular endothelial cells (HMVEC-L).

  4. Oncology - Breast Cancer: MDA cells adhering to TNF-alpha stimulated HUVECs under shear flow.

So now you know the features and benefits of the Kima Pump and how it works.

But, if you still have questions or want to learn more, check out our website, at wearecellix.com, or contact us at info@wearecellix.com.








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