How to change the SIM for Inish Analyser?
Introduction
As the name says, automated cell counters like the Inish Analyser are devices that can count cells automatically. It is a pretty straightforward process - you load your sample into the cell counter, which pumps your cell sample through through the device. The Inish Analyser counts cells, quantifies cell viability and can even predict transfection efficiencies based on disturbances to the membrane. The technology behind the Inish Analyser is microfluidic impedance spectroscopy.
The main features and benefits of the Inish Analyser include:
Label-free: No fluorescent stains or dyes.
Single-cell Analysis: Every cell is counted!
High-throughput: Analyse thousands of cells per second.
Compatible with a wide variety of cell samples: cell lines (e.g. Jurkats, CHO cells etc.), primary cells (e.g. PBMCs), stem cells, yeast cells, sperm cells.
Fast Set-up & Analysis: 90 seconds including sample preparation.
Easy-to-use Benchtop Instrument: fits inside standard biosafety cabinet and operated by simple touchscreen workflow.
Compatible with FCS & CSV file format: easily exportable.
This method offers reliable cell counting with high precision and throughput.
What type of cells can Inish analyse?
The Inish Analyser analysis a wide variety of cells such as T-cells, PBMCs, stem cells, cell lines (Jurkat, CHO etc.), yeast cells and sperm cells.
The Inish Analyser comes with one Sample Injector Module. The recommended SIM is SIM60 as it is compatible with most cell types but with every order the customer gets a choice of SIM depending on their needs. SIM30 is suitable for analysing cells of 2µm - 12µm in size and
SIM60 is suitable for analysing cells of 6µm - 25µm. SIMs are interchangeable - so you don't have to buy a whole new Inish Analyser for each cell size range; just swap out the SIM as shown in the image below.
Swapping SIMs
Swapping SIMS is easy and takes just a couple of minutes.
To get started, first, ensure the power is switched off and then remove the sleeve connecting the SIM to the main base of the Inish Analyser. Once you do that, you will see three connections:
Input & Output electrical cables to the microfluidic impedance chip for detection and analysis of the cells.
Power cable
Pump pneumatic tubing connecting the pump to the sample tube and waste tube, ensuring accurate flow of the sample through the microfluidic chip.
Follow the following steps in order to remove the sim:
Start with unscrewing the input & output electrical connections.
Then, unplug the power cable.
The next step is to disconnect the blue pneumatic tubings, you can do this by pressing gently on the black connector to release and remove the tubing.
Use the allen key provided to unscrew the SIM60 connected to the base.
Remove the tape over the screw on the SIM30 and use the two posts to align the SIM30 on the base of the Inish Analyser and screw it in place.
To reconnect to the Inish base unit;
Plug in the two blue pneumatic tubings as shown.
Plug in the power cable.
Screw the input & output cables in place
Now replace the sleeve covering the connections.
And that’s it!
In just a few minutes your SIM60 for SIM30 on the Inish Analyser will be ready for you to start your experiments.
Click here to learn more about Cellix’s Inish Analyser.
Click here for Inish Analyser FAQ and if you have any more questions, would like an online demo or if you’d like a quote, contact us now.