YEAST CELLS

Yeast cells are a popular choice in biologics for the manufacture of cell products.  Yeast cells as "cell factories" have numerous advantages including their fast growth; the ability to easily manipulate their genetics and their compatibility with high-density large-scale fermentation processes for biochemical production. A reliable cell count and cell viability are crucial metrics for many regulatory aspects of biomanufacturing:

  • Safety, efficacy, purity, potency, identity, quality

  • Oversight of both product and process

  • Quality control of source materials, intermediates, and product

  • Reproducibility of lots

  • Comparability after manufacturing change

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WHAT DO I NEED TO GET STARTED?

Materials

The Inish Analyser does not require any expensive consumables such as slides or cartridges.  Instead we use affordable standard tubes (500 µL), available from Cellix.  Sample preparation is very simple - just using a pipette. 

In general, you will need the following to execute Cell Counting & Viability and Transfection Efficiency assays:

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Cell Sample Preparation

In this example, we use  Saccharomyces cerevisiae.  Culture the yeast cells in the recommended medium / PBS.  Ensure that the cells are in suspension.  Recommended cell concentration is 50,000 - 2,000,000 cells/mL.  Vortex cells or pipette cells up and down to ensure a homogenous solution.

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Results of Cell Counting & Viability Assay

Cell Counting & Viability of Yeast cells (Saccharomyces cerevisiae)
Cell Counting & Viability of Yeast cells (Saccharomyces cerevisiae)

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Cell Counting & Viability of Yeast cells (Saccharomyces cerevisiae)
Cell Counting & Viability of Yeast cells (Saccharomyces cerevisiae)

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