Yeast cells are a popular choice in biologics for the manufacture of cell products. Yeast cells as "cell factories" have numerous advantages including their fast growth; the ability to easily manipulate their genetics and their compatibility with high-density large-scale fermentation processes for biochemical production. A reliable cell count and cell viability are crucial metrics for many regulatory aspects of biomanufacturing:
Safety, efficacy, purity, potency, identity, quality
Oversight of both product and process
Quality control of source materials, intermediates, and product
Reproducibility of lots
Comparability after manufacturing change
WHAT DO I NEED TO GET STARTED?
The Inish Analyser does not require any expensive consumables such as slides or cartridges. Instead we use affordable standard tubes (500 µL), available from Cellix. Sample preparation is very simple - just using a pipette.
In general, you will need the following to execute Cell Counting & Viability and Transfection Efficiency assays:
Cell Sample Preparation
In this example, we use Saccharomyces cerevisiae. Culture the yeast cells in the recommended medium / PBS. Ensure that the cells are in suspension. Recommended cell concentration is 50,000 - 2,000,000 cells/mL. Vortex cells or pipette cells up and down to ensure a homogenous solution.