Cell Counting & Viability and Transfection Efficiencies of stem cells are key metrics for R&D applications and Quality Control.
Applications include tissue regeneration or stem cell therapy for blood disorders, cardiovascular and brain diseases. These metrics are important because they provide information about the overall health of the cells during experiments.
WHAT DO I NEED TO GET STARTED?
The Inish Analyser does not require any expensive consumables such as slides or cartridges. Instead we use affordable standard tubes (500 µL), available from Cellix. Sample preparation is very simple - just using a pipette.
In general, you will need the following to execute Cell Counting & Viability and Transfection Efficiency assays:
Cell Sample Preparation
In this example, we use Human Bone Marrow-Derived Mesencyhmal Stem cells. Culture Mesenchymal Stem Cells (Human Bone Marrow-Derived MSCs from ATCC: PCS-500-012) in the recommended medium Alpha MEM with Glutamax (GibcoTM 32561), Fetal Bovine Serium 10% (Sigma F4135) and FGF2 (20ng/mL, R&D Systems AFL233). Trypsinise the cells and resuspend the cells in growth media. Centrifuge the cells at 50g for 8 minutes. After centrifugation, resuspend the cells in growth media to ensure the cells are in suspension. Recommended cell concentration is 50,000 - 2,000,000 cells/mL. Vortex cells or pipette cells up and down to ensure a homogenous solution.