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PRIMARY T-CELLS

Cell Counting & Viability and Transfection Efficiencies of primary T-cells from whole blood are key metrics for R&D applications and Quality Control. Applications include biomanufacturing of cell-based products for gene therapy (including CAR T-cell therapies), cancer cell research and single-cell sequencing of primary cells  These metrics are important because they provide information about the overall health of the cells during experiments.

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WHAT DO I NEED TO GET STARTED?

Materials

​The Inish Analyser does not require any expensive consumables such as slides or cartridges.  Instead we use affordable standard tubes (500 µL), available from Cellix.  Sample preparation is very simple - just using a pipette. 

In general, you will need the following to execute Cell Counting & Viability and Transfection Efficiency assays:​

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Cell Sample Preparation

Isolate primary T-cells from whole blood using T-cell isolation kit from Stemcell Technologies.  Ensure the cells are in suspension.  Recommended cell concentration is 50,000 - 2,000,000 cells/mL.  Vortex cells or pipette cells up and down to ensure a homogenous solution.​

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Results of Cell Counting & Viability Assay

What do I need to get started?

DOWNLOADS

Downloads
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