PRIMARY T-CELLS

Cell Counting & Viability and Transfection Efficiencies of primary T-cells from whole blood are key metrics for R&D applications and Quality Control. Applications include biomanufacturing of cell-based products for gene therapy (including CAR T-cell therapies), cancer cell research and single-cell sequencing of primary cells  These metrics are important because they provide information about the overall health of the cells during experiments.

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WHAT DO I NEED TO GET STARTED?

Materials

The Inish Analyser does not require any expensive consumables such as slides or cartridges.  Instead we use affordable standard tubes (500 µL), available from Cellix.  Sample preparation is very simple - just using a pipette. 

In general, you will need the following to execute Cell Counting & Viability and Transfection Efficiency assays:

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Cell Sample Preparation

Isolate primary T-cells from whole blood using T-cell isolation kit from Stemcell Technologies.  Ensure the cells are in suspension.  Recommended cell concentration is 50,000 - 2,000,000 cells/mL.  Vortex cells or pipette cells up and down to ensure a homogenous solution.

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Results of Cell Counting & Viability Assay

Cell Counting & Viability of Primary T-cells (isolated from whole blood)
Cell Counting & Viability of Primary T-cells (isolated from whole blood)

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Cell Counting & Viability of Primary T-cells (isolated from whole blood)
Cell Counting & Viability of Primary T-cells (isolated from whole blood)

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