PRIMARY T-CELLS
Cell Counting & Viability and Transfection Efficiencies of primary T-cells from whole blood are key metrics for R&D applications and Quality Control. Applications include biomanufacturing of cell-based products for gene therapy (including CAR T-cell therapies), cancer cell research and single-cell sequencing of primary cells These metrics are important because they provide information about the overall health of the cells during experiments.
WHAT DO I NEED TO GET STARTED?
Materials
​The Inish Analyser does not require any expensive consumables such as slides or cartridges. Instead we use affordable standard tubes (500 µL), available from Cellix. Sample preparation is very simple - just using a pipette.
In general, you will need the following to execute Cell Counting & Viability and Transfection Efficiency assays:​
Cell Sample Preparation
Isolate primary T-cells from whole blood using T-cell isolation kit from Stemcell Technologies. Ensure the cells are in suspension. Recommended cell concentration is 50,000 - 2,000,000 cells/mL. Vortex cells or pipette cells up and down to ensure a homogenous solution.​
Results of Cell Counting & Viability Assay
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