Probing cells using nanoparticles
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Biochip-based assay development to advance the potential of nanoparticles
The BioNanoInteract Grant Project
Cellix worked on the development of a novel microfluidic biochip and assay development to enable researchers to investigate cellular uptake of nanoparticles in endothelial cells under defined shear stresses (flow rates) mimicking those found in vivo. Below are a sample of some of the results.
This project aims to use the complementary state of the art imaging techniques to probe all aspects of the interaction of nanoparticles with cells, from determination of the initial portal of entry, to the formation of endosomes and the onward trafficking of the nanoparticles to their final sub -cellular localisations or exocytosis.
On a global level, the aim is to develop a panel of nanoparticle-based live cell markers for each of the stages of cell cycle, for use in quantitative assays in High Content Analysis, and as probes for disease or cell cycle disregulation in the longer term.
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Cellular uptake of CdTe QDs by human endothelial cells under various shear stresses.
Cellular uptake of silica NPs by human endothelial cells under various shear stresses.
CdTe QDs, but not silica NPs, influence viability of human endothelial cells.
*SFI provides grants for researchers from around the world who wish to relocate to Ireland and those already based in Ireland, for outstanding investigators, for conferences and symposia, and for collaboration with industry. The majority of SFI awards are in the fields of science and engineering underpinning three Government-prioritised industrial sectors: biotechnology (Bio), information and communications technology (ICT) and sustainable energy and energy-efficient technologies.
At a smaller scale SFI also supports a broad range of disciplines in Science, Mathematics and Engineering via its Research Frontiers Programme. Proposals are evaluated in open competitions via a combination of international peer review and strategic fit with SFI’s mission.